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˸w򵰰3(HMGB3)zyԇ
a(chn)ƷQ
˸w򵰰3(HMGB3)zyԇ
ضȣ
2-8
a(chn)Ʒ̖
RXM100493H
a(chn)ƷҎ(gu)
96T/48T
a(chn)Ʒ
100
a(chn)Ʒr
h
ƷԔa(chn)Ʒfʽ(w)h
(yng)N
zy 1.5625ng/mL-50ng/mL
ҺwM֑(yng)壬oнM֑(yng)obƓp
; zyѪѪ{(x)B(yng)ҺȘӱ˸w򵰰3(HMGB3)ĝȡ
ԭ ԇвpwAø“(lin)ԇ򞣨ELISAA(y)˸w򵰰3(HMGB3)w࿹w΢ø(bio)˸w򵰰3(HMGB3)У(zhn)Ʒʹyӱټؘ(bio)ӛw(jng)^cϴټHRPż“(lin)H(jng)^cϴ죬ȥδY(ji)ϵĽM΢װγɹ࿹w-ԭ-ؘ(bio)ӛw-HøĊAď(f)ӵABHRP߻£a(chn){(ln)ɫa(chn)ڽKֹҺ2Mᣩ£KD(zhun)Sɫø(bio)x450nmLϜyȣODֵȣODֵcyƷ˸w򵰰3(HMGB3)ĝP(gun)MУ(zhn)ƷӋӱ˸w򵰰3(HMGB3)ĝ
خ ԇReȻؽM˸w򵰰3(HMGB3)cY(ji)(gu)o
؏(f) (ni)׃ϵ(sh)<10%g׃ϵ(sh)<15%
85%-115%֮g
ԇԂԇ 1ø(bio)x450nm2Һһ^3sˮ4ϴƿԄϴC(j)537ˮԡ偻6500ml Ͳ
ԇͽMֶȻ֏(f)Ҝ(bio)(zhn)Ʒ|(zh)Ʒ͘Ʒh(f)ס1ǰfķƺԇиNMֵĹҺ2XȡlʣİlԷܷŻر3O(sh)Ø(bio)(zhn)Ʒ0 ֵհ׿׺͘ӱ(bio)(zhn)Ʒ׸ӲͬȵĘ(bio)(zhn)Ʒ 50L0 ֵ׼ӘӱϡҺ 50Lհ׿ײӣӱ׼Ӵyӱ 50L4հ׿⣬(bio)(zhn)Ʒ0 ֵ׺͘ӱػw 50L5÷Ĥwס(yng)37ˮԡ偻ܹ 30min6_ĤȥҺwˮĸÿ׼ӝMϴҺo 20S˦ȥϴҺˮĸ؏(f) 5 ʹԄϴC(j)ՈϴC(j)M(jn)ϴӽ 30s ijߙzyľϴY(ji)ӵǰҪڸɃмļĸɷ(yng)7հ׿ÿ׼ HRP (bio)ӛH 50L8÷Ĥwס(yng)壬37ˮԡ偻ܹ 30min9؏(f)E 610 A B 1:1 weֻϣпмҺ 100L÷Ĥwס(yng)壬37ˮԡ偻ܹ 15min11п׼KֹҺ 50Lø(bio)x 450nm LxȡȣODֵ
ע 1ԇеĽMҪٴʹՈ_һʹ֮]бȾ2ø(bio)δδʹҪ֔(jn)ӛܷŵ 2-8汣
} ن}(bio)(zhn)ԭ(yng)ߣ1.ԇƽrg򡪡 2 СrҜƽ2.ȡϴ첻ֵ֡ȡϴ3.Һ_zУҺچ}ܶȵͿԭ(yng)ߣ1.ϴ첻֡fҪϴͽ2.򲻳ֺȡԇ㡪ֻȡԇ3.؏(f)^͸ĤʹüӘҪQµ^ʹµķģ4.ӘӲ_zУҺۆ}O.DֵͿԭ(yng)ߣ1.ÿ׼ӵԇ_УҺ_ԇ2.rg_CĜrg3.ضȲ_ԇҪƽҜزC(zhn)_Ĝض4.ø(bio)ӛʧЧͨ^ø(bio)ӛ͵ɫѸ@F(xin)z5.]мKֹҺfEKֹҺ6.x(sh)rgx(sh)f]x(sh)rg(ni)x(sh)܆}ӱֵԭ(yng)ߣ1._Ęӱ淽ʽ_ӱʹrӱM(jn)Ќ2._Ęӱռ̎ȡ_Ęӱռ̎3.y|(zh)ژӱк͡ʹrӱ؏(f)
• ԇЃHоʹ猢R\κ;ҹ˾ˮa(chn)Ć}ؓ(f)؟(z)಻Г(dn)κη؟(z)

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