a(chn)ƷB˸μLӣHGFELISAԇеã
cΔ(sh)18 l(f)rg2025/4/30 10:55:05t(y)W(xu)оR\I(lng),
ø(lin)ԇELISA
һNV(yng)õĸ`șzyg(sh).
,
˸μLӣHGFELISAԇ
ڸK [о
Mޏ(f)ȷҪ,
ɞкt(y)zyеP(gun)I.
ČԔBHGF ELISAԇе
ԭ ÙC (yng)I(lng)
t(y)W(xu)оеҪx.
һʲôHGF
μLӣHepatocyte Growth Factor, HGFһNg|(zh)ڵĶܼ,ܴMμֳ.HGFW(xu)hֹڴ,߀cw Mޏ(f) ѪɵȶNͲ^.
HGFwc-MetһNҰἤøw,(dng)HGFcc-MetY(ji)Ϻ,ɼ̖ͨ·PI3K/AKT MAPKȣ,M{(dio)ؼֳ \.
HGFڸK YD(zhun) wS^аl(f)]P(gun)I,zyڼCо ˎ_l(f)R\Ҫx.
˸μL(HGF)ELISAԇк
˸μL(HGF)ELISAԇһNø(lin)ԇELISAԭęzy,ڶzyѪ Ѫ{ B(yng)ҺM{еHGFˮƽ.
1. zyԭ
ELISAԇͨpwAķ,E£
װA(y)ȰHGFw,ڲ@ӱеHGF.
yӱ,HGFcwY(ji).
zyؘ(bio)ӛĶ,γ“w-HGF-”(f).
@ɫ^øHRP(bio)ӛùH,TMB@ɫ.
yø(bio)xzyȣODֵ,ӋHGF.
2. ԇнM
A(y)wװ
HGF(bio)(zhn)Ʒ(bio)(zhn)
ؘ(bio)ӛęzyw
HRP(bio)ӛùH
ϴ쾏_Һ
@ɫTMB
KֹҺᣩ
3. zyc
`ȣəzypg/mLeHGF.
خԣHReHGF,cӽ淴(yng).
㣺mڌҳҎ(gu)zy.
؏(f)Ժã(ni)g׃ϵ(sh),Y(ji)(wn)ɿ.
˸μL(HGF)ELISAԇе
˸μL(HGF)ELISAԇڶI(lng)l(f)]Ҫ,Ҫ
1. Kо
HGFMμl(f)F(xin),ڸΓp wS ӲȼоP(gun)Ҫ.磺
ԸΓpHGFˮƽ,Mμޏ(f).
wSHGFƸǠ,pwSMչ.
2. Yо
HGF/c-Met̖ͨ·ڶN[ΰ ΰ ٰ^ȼ,M[uD(zhun).,HGFzyڣ
[(bio)־uYߵHGFˮƽ,A(y)yA(y).
ˎаl(f)c-MetƄᣩįЧu.
3. Mޏ(f)ct(y)W(xu)
HGFܴMѪ ,ڄ(chung)ޏ(f) ɼίI(lng)Нڑ(yng)Ãrֵ.
4. Ѫܼ
HGFɴMѪ܃(ni)Ƥֳ,ȱѪK ܄}.
5. װYc{(dio)(ji)
HGFп,ԼL(fng)P(gun)(ji)ףпܰl(f)]o.
ģ˸μL(HGF)ELISAԇеRx
˸μL(HGF)ELISAԇвHڿ,߀R\оҪrֵ
1. \cO(jin)y
ΰHGFˮƽc[MչP(gun),o\ָ(bio).
ԸβO(jin)yHGF׃,uwS̶.
ѪܼHGFˮƽcļpޏ(f)P(gun).
2. A(y)u
YߣHGF߱_ʾA(y).
ֲHGFˮƽɷӳֲr.
3. ˎ_l(f)cЧu
wSˎHGFؽMHGFįЧO(jin)y.
ˎc-MetƄRԇ.
˸μLӣHGFELISAԇ
һN` خԵęzy,
ڸK Y Mޏ(f)
ѪܼȶI(lng)ЏVđ(yng)Ãrֵ.
HˆTṩҪČֶ,
߀R\ˎ_l(f)аl(f)]P(gun)I.
(qun)УȪƼ˾ | }ICP18019542̖ | g(sh)֧֣xW(wng)
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